A novel procedure for the rapid purification of plastocyanin from pea (Pisum sativum) leaves.

نویسندگان

  • A R Ashton
  • L E Anderson
چکیده

Plastocyanin is soluble at high concentrations (greater than 3 M) of (NH4)2SO4 but under these conditions will adsorb tightly to unsubstituted Sepharose beads. This observation was utilized to purify plastocyanin from pea (Pisum sativum) in two chromatographic steps. Sepharose-bound plastocyanin was eluted with low-ionic-strength buffer and subsequently purified to homogeneity by DEAE-cellulose chromatography.

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عنوان ژورنال:
  • The Biochemical journal

دوره 193 1  شماره 

صفحات  -

تاریخ انتشار 1981